ELISA/assay

Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit

MBS2507534

48-Strip-Wells

410 USD

ELISA Kit

Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit

NOS1/nNOS

nitric oxide synthase, brain isoform 1; Nitric oxide synthase, brain; nitric oxide synthase, brain; NOS type I; constitutive NOS; neuronal NOS; peptidyl-cysteine S-nitrosylase NOS1; nitric oxide synthase 1 (neuronal); Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS

NOS1/nNOS

NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS

NOS1_HUMAN

Rat

1434

This kit recognizes natural and recombinant Rat NOS1/nNOS. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Rat NOS1/nNOS is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NOS1/nNOS concentrations in serum, plasma and other biological fluids.

. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NOS1/nNOS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NOS1/nNOS. You can calculate the concentration of NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.

10835173

NP_000611.1

NM_000620.4

P29475

164,779 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459)

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 12q24.22. Cellular Component: photoreceptor inner segment; protein complex; cytoskeleton; sarcoplasmic reticulum; mitochondrion; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft. Molecular Function: calmodulin binding; protein binding; sodium channel regulator activity; FAD binding; nitric-oxide synthase activity; FMN binding; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding. Biological Process: negative regulation of serotonin uptake; interaction with host; negative regulation of calcium ion transport; striated muscle contraction; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; blood coagulation; nitric oxide biosynthetic process; multicellular organismal response to stress; nitric oxide mediated signal transduction; myoblast fusion; neurotransmitter biosynthetic process. Disease: Pyloric Stenosis, Infantile Hypertrophic, 1

48-Strip-Wells

410 USD

96-Strip-Wells

490