ELISA/assay

Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

MBS2507522

24-Strip-Wells

215 USD

ELISA Kit

Mouse GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

[GDNF]

[glial cell line-derived neurotrophic factor isoform 4 preproprotein; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor; ATF]

[GDNF]

[GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF]

GDNF_HUMAN

Mouse

202

This kit recognizes Mouse GDF11 in samples. No significant cross-reactivity or interference between Mouse GDF11and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level MouseGDF11were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MouseGDF11 were testedon 3 different plates, 20replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse GDF11 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GDF11. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of GDF11. You can calculate the concentration of GDF11 in the samples by comparing the OD of the samples to the standard curve.

299473781

NP_001177398.1

NM_001190469.1

P39905

18,123 Da

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM1 Interactions Pathway (105697); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915); Spinal Cord Injury Pathway (739007)

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5p13.1-p12. Cellular Component: extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; receptor binding. Biological Process: positive regulation of dopamine secretion; nervous system development; axon guidance; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; positive regulation of cell differentiation; postsynaptic membrane organization; neural crest cell migration; metanephros development; neurite development; negative regulation of apoptosis. Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590