ELISA/assay

Human PF4V1 (Platelet Factor 4 Variant 1) ELISA Kit

MBS2507520

48-Strip-Wells

410 USD

ELISA Kit

Human PF4V1 (Platelet Factor 4 Variant 1) ELISA Kit

PF4V1

platelet factor 4 variant; Platelet factor 4 variant; platelet factor 4 variant; C-X-C motif chemokine 4; PF4alt; PF4var1; platelet factor 4, variant 1 (PF4-like); platelet factor 4 variant 1; C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1Cleaved into the following 3 chains:Platelet factor 4 variant(4-74); Platelet factor 4 variant(5-74); Platelet factor 4 variant(6-74)

PF4V1

PF4V1; PF4V1; PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1; CXCL4V1; SCYB4V1

PF4V_HUMAN

Human

104

This kit recognizes natural and recombinant Human PF4V1. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: The minimum detectable dose of Human PF4V1 is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PF4V1 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PF4V1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PF4V1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PF4V1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PF4V1. You can calculate the concentration of PF4V1 in the samples by comparing the OD of the samples to the standard curve.

4505735

NP_002611.1

NM_002620.2

P10720

11,553 Da

Cell Surface Interactions At The Vascular Wall Pathway (106062); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Common Pathway (106060); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)

PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Chemokine; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q21. Cellular Component: extracellular space. Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; inflammatory response; regulation of cell proliferation

48-Strip-Wells

410 USD

96-Strip-Wells

490