Mouse FOXO1 (Forkhead Box Protein O1) ELISA Kit | MyBioSource MBS2507477 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse FOXO1 (Forkhead Box Protein O1) ELISA Kit

catalog :

MBS2507477

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2507477

products type :

ELISA Kit

products full name :

Mouse FOXO1 (Forkhead Box Protein O1) ELISA Kit

products short name :

[FOXO1]

other names :

[forkhead box protein O1; Forkhead box protein O1; forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead box O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma]

products gene name :

[FOXO1]

other gene names :

[FOXO1; FOXO1; FKH1; FKHR; FOXO1A; FKHR; FOXO1A]

uniprot entry name :

FOXO1_HUMAN

reactivity :

Mouse

sequence length :

655

specificity :

This kit recognizes Mouse FGF21 in samples. No significant cross-reactivity or interference between Mouse FGF21and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level MouseFGF21were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MouseFGF21 were testedon 3 different plates, 20replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse FGF21 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse FGF21. You can calculate the concentration of Mouse FGF21 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

9257222

ncbi acc num :

NP_002006.2

ncbi gb acc num :

NM_002015.3

uniprot acc num :

Q12778

ncbi mol weight :

69,662 Da

ncbi pathways :

AGE/RAGE Pathway (698754); AKT Phosphorylates Targets In The Nucleus Pathway (106476); AKT-mediated Inactivation Of FOXO1A Pathway (106333); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adaptive Immune System Pathway (366160); Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); B Cell Receptor Signaling Pathway (198909)

ncbi summary :

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

uniprot summary :

FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain. Protein type: Oncoprotein; Transcription factor; DNA-binding; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 13q14.1. Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding. Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular response to starvation; negative regulation of stress-activated MAPK cascade; negative regulation of fat cell differentiation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of protein catabolic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; autophagy; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis. Disease: Rhabdomyosarcoma 2

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590