catalog number :
MBS2507377
products type :
ELISA Kit
products full name :
Mouse IgG1 (Immunoglobulin G1) ELISA Kit
products short name :
[IgG1]
other names :
[IgG1, partial]
products gene name :
[IgG1]
specificity :
This kit recognizes natural and some recombinant Mouse IgG1 . No significant crossreactivity or interference between Mouse IgG1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50?g/mL. Sensitivity: Min: 0.469?g/mL; Max: 50?g/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse IgG1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse IgG1 . Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse IgG1 , biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG1 . The concentration of Mouse IgG1 in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
ACK87036.1
size5 :
10x96-Strip-Wells