Human PCI (Procollagen I) ELISA Kit | MyBioSource MBS2507363 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PCI (Procollagen I) ELISA Kit

catalog :

MBS2507363

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2507363

products type :

ELISA Kit

products full name :

Human PCI (Procollagen I) ELISA Kit

products short name :

[PCI]

other names :

[plasma serine protease inhibitor preproprotein; Plasma serine protease inhibitor; plasma serine protease inhibitor; acrosomal serine protease inhibitor; plasminogen activator inhibitor III; plasminogen activator inhibitor-3; protein C inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5; Acrosomal serine protease inhibitor; Plasminogen activator inhibitor 3; PAI-3; PAI3; Protein C inhibitor; PCI; Serpin A5]

products gene name :

[PCI]

other gene names :

[SERPINA5; SERPINA5; PCI; PAI3; PAI-3; PCI-B; PROCI; PLANH3; PCI; PLANH3; PROCI; PAI-3; PAI3; PCI]

uniprot entry name :

IPSP_HUMAN

reactivity :

Human

sequence length :

406

specificity :

This kit recognizes natural and recombinantHumanPCI. No significant cross-reactivity or interference between HumanPCI and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPCIconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PCI. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PCIand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PCI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PCI.You can calculate the concentration of PCIin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

194018472

ncbi acc num :

NP_000615.3

ncbi gb acc num :

NM_000624.5

uniprot acc num :

P05154

ncbi mol weight :

45,675 Da

ncbi pathways :

Common Pathway (106060); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Intrinsic Pathway (106059)

ncbi summary :

The protein encoded by this gene is a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. This gene is one in a cluster of serpin genes located on the q arm of chromosome 14. This family member is a glycoprotein that can inhibit several serine proteases, including protein C and various plasminogen activators and kallikreins, and it thus plays diverse roles in hemostasis and thrombosis in multiple organs. [provided by RefSeq, Aug 2012]

uniprot summary :

SERPINA5: Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary- type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as an hydrophobic hormone carrier by its binding to retinoic acid. Belongs to the serpin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 14q32.1. Cellular Component: extracellular space; protein complex; membrane; extracellular region; acrosomal membrane; external side of plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; acrosin binding; retinoic acid binding; glycosaminoglycan binding; protease binding; phosphatidylcholine binding. Biological Process: fusion of sperm to egg plasma membrane; spermatogenesis; blood coagulation; lipid transport

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590