catalog number :
MBS2507358
products type :
ELISA Kit
products full name :
Human IL-8 (Interleukin 8) ELISA Kit
products short name :
[IL-8]
other names :
[interleukin-8; Interleukin-8; interleukin-8; C-X-C motif chemokine 8; IL-8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; chemokine (C-X-C motif) ligand 8; Alveolar macrophage chemotactic factor I; AMCF-I; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8]
products gene name :
[IL-8]
other gene names :
[IL8; CXCL8; CXCL8; AMCF-I; IL8; IL-8; AMCF-I]
uniprot entry name :
IL8_PIG
specificity :
This kit recognizes natural and recombinant Human IL-8. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-8. You can calculate the concentration of IL-8 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_999032.1
ncbi gb acc num :
NM_213867.1
ncbi mol weight :
11,633 Da
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Cellular Senescence Pathway (1125456); Cellular Responses To Stress Pathway (1125444); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1124657); Chemokine Signaling Pathway (98758)
uniprot summary :
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
size5 :
10x96-Strip-Wells