Human MUC5B (Mucin 5 Subtype B) ELISA Kit | MyBioSource MBS2507356 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MUC5B (Mucin 5 Subtype B) ELISA Kit

catalog :

MBS2507356

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2507356

products type :

ELISA Kit

products full name :

Human MUC5B (Mucin 5 Subtype B) ELISA Kit

products short name :

[MUC5B]

other names :

[mucin-5B; Mucin-5B; mucin-5B; cervical mucin MUC5B; high molecular weight salivary mucin MG1; mucin 5, subtype B, tracheobronchial; sublingual gland mucin; mucin 5B, oligomeric mucus/gel-forming; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B, tracheobronchial; Sublingual gland mucin]

products gene name :

[MUC5B]

other gene names :

[MUC5B; MUC5B; MG1; MUC5; MUC9; MUC-5B; MUC5; MUC-5B]

uniprot entry name :

MUC5B_HUMAN

reactivity :

Human

sequence length :

5762

specificity :

This kit recognizes Human MUC2 in samples. No Significant cross-reactivity or interference between Human MUC2 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20 ng/mL. Sensitivity: 0.19 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MUC2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MUC2 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MUC2. You can calculate the concentration of Human MUC2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

301172750

ncbi acc num :

NP_002449.2

ncbi gb acc num :

NM_002458.2

uniprot acc num :

Q9HC84

ncbi mol weight :

596,340 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Termination Of O-glycan Biosynthesis Pathway (530748)

ncbi summary :

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

uniprot summary :

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741). Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; Golgi lumen. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification. Disease: Pulmonary Fibrosis, Idiopathic

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590