Monkey ApoC3(Apolipoprotein C3)ELISA Kit | MyBioSource MBS2507355 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey ApoC3(Apolipoprotein C3)ELISA Kit

catalog :

MBS2507355

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2507355

products type :

ELISA Kit

products full name :

Monkey ApoC3(Apolipoprotein C3)ELISA Kit

products short name :

ApoC3

other names :

apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III; Apolipoprotein C3

products gene name :

ApoC3

other gene names :

APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III

uniprot entry name :

APOC3_HUMAN

reactivity :

Monkey

sequence length :

specificity :

This kit recognizes natural and recombinant Monkey ApoC3. No significant cross-reactivity or interference between Monkey ApoC3 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: The minimum detectable dose of Monkey ApoC3 is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey ApoC3 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoC3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoC3. You can calculate the concentration of ApoC3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4557323

ncbi acc num :

NP_000031.1

ncbi gb acc num :

NM_000040.1

uniprot acc num :

P02656

ncbi mol weight :

10,852 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

ncbi summary :

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

uniprot summary :

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Secreted; Secreted, signal peptide; Lipid-binding. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity. Biological Process: response to drug; response to peptide hormone stimulus; phototransduction, visible light; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of receptor-mediated endocytosis; negative regulation of lipoprotein lipase activity; triacylglycerol mobilization; negative regulation of lipid metabolic process; inflammatory response; retinoid metabolic process; response to nutrient; negative regulation of fatty acid biosynthetic process. Disease: Apolipoprotein C-iii Deficiency

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

475

more info or order :

MyBioSource product webpage