catalog number :
MBS2507164
products type :
ELISA Kit
products full name :
Human GDF10 (Growth Differentiation Factor 10) ELISA Kit
products short name :
[GDF10]
other names :
[GDF10 protein; Bone morphogenetic protein 3B; bone morphogenetic protein 3B; BIP; GDF-10; bone-inducing protein; growth differentiation factor 10; Bone-inducing protein; BIP; Growth/differentiation factor 10; GDF-10]
products gene name :
[GDF10]
other gene names :
[GDF10; GDF10; BMP3B; BMP-3b; BMP3B; BMP-3B; BIP; GDF-10]
uniprot entry name :
BMP3B_HUMAN
specificity :
This kit recognizes Human GBP4 in samples. No Significant cross-reactivity or interference between Human GBP4 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GBP4 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GBP4 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GBP4 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GBP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GBP4. You can calculate the concentration of Human GBP4 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAH28237.1
ncbi mol weight :
53,122 Da
ncbi pathways :
Adipogenesis Pathway (198832)
ncbi summary :
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]
uniprot summary :
GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.22. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: fat cell differentiation; regulation of apoptosis; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; cell development; skeletal development; growth
size5 :
10x96-Strip-Wells