catalog number :
MBS2507163
products type :
ELISA Kit
products full name :
Mouse ET-1 (Endothelin 1) ELISA Kit
products short name :
[ET-1]
other names :
[endothelin-1; Endothelin-1; endothelin-1; ET-1; gene for endothelin; preproendothelin-1; Preproendothelin-1; PPET1]
products gene name :
[ET-1]
other gene names :
[EDN1; EDN1; PPET1; ET-1; PPET1]
uniprot entry name :
EDN1_CANFA
specificity :
This kit recognizes natural and recombinant Mouse ET-1. No significant cross-reactivity or interference between Mouse ET-1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 7.813-500pg/mL. Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse ET-1 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ET-1. You can calculate the concentration of ET-1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001002956.1
ncbi gb acc num :
NM_001002956.1
ncbi mol weight :
22,977 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1128453); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1128451); Disease Pathway (1128149); G Alpha (q) Signalling Events Pathway (1128706); GPCR Downstream Signaling Pathway (1128702); GPCR Ligand Binding Pathway (1128452); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1128719); HIF-1 Signaling Pathway (695209); Melanogenesis Pathway (84142); Melanogenesis Pathway (504)
uniprot summary :
Endothelins are endothelium-derived vasoconstrictor peptides.
size5 :
10x96-Strip-Wells