catalog number :
MBS2507055
products type :
ELISA Kit
products full name :
Porcine IL-9 (Interleukin 9) ELISA Kit
products short name :
[IL-9]
other names :
[interleukin-9; Interleukin-9; interleukin-9; T-cell growth factor p40; cytokine P40; homolog of mouse T cell and mast cell growth factor 40; p40 T-cell and mast cell growth factor; p40 cytokine; interleukin 9; Cytokine P40; T-cell growth factor P40]
products gene name :
[IL-9]
other gene names :
[IL9; IL9; P40; HP40; IL-9; IL-9]
uniprot entry name :
IL9_HUMAN
specificity :
This kit recognizes natural and recombinant Porcine IL-9. No significant cross-reactivity or interference between Porcine IL-9 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine IL-9 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-9. You can calculate the concentration of IL-9 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_000581.1
ncbi gb acc num :
NM_000590.1
ncbi mol weight :
15,909 Da
ncbi pathways :
Asthma Pathway (83120); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL-9 Signaling Pathway (198883); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); TCR Signaling Pathway (198862)
ncbi summary :
The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
uniprot summary :
IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space. Molecular Function: growth factor activity; interleukin-9 receptor binding; cytokine activity. Biological Process: positive regulation of cell proliferation; immune response; inflammatory response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process
size5 :
10x96-Strip-Wells