ELISA/assay

Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

MBS2506881

24-Strip-Wells

190 USD

ELISA Kit

Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

[VEGFR1/FLT1]

[vascular endothelial growth factor receptor 1 isoform 4; Vascular endothelial growth factor receptor 1; vascular endothelial growth factor receptor 1; fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; fms-related tyrosine kinase 1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor]

[VEGFR1/FLT1]

[FLT1; FLT1; FLT; FLT-1; VEGFR1; VEGFR-1; FLT; FRT; VEGFR1; VEGFR-1; FLT-1; FLT]

VGFR1_HUMAN

Human

541

This kit recognizes natural and some recombinant Human VEGFR1/FLT1 . No significant cross-reactivity or interference between Human VEGFR1/FLT1 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: Min: 75pg/mL; Max: 8000pg/mL

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human VEGFR1/FLT1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human VEGFR1/FLT1 . Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human VEGFR1/FLT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human VEGFR1/FLT1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR1/FLT1 . The concentration of Human VEGFR1/FLT1 in samples can be calculated by comparing the OD of the samples with the standard curve.

229892302

NP_001153503.1

NM_001160031.1

P17948

41,175 Da

Angiogenesis Pathway (198772); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Glypican 1 Network Pathway (138010); HIF-1 Signaling Pathway (695200)

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis. Protein type: EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; VEGFR family. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; plasma membrane; endosome; receptor complex. Molecular Function: identical protein binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: cell migration; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; blood vessel morphogenesis; embryonic morphogenesis; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805