Human MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit | MyBioSource MBS2506867 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit

catalog :

MBS2506867

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2506867

products type :

ELISA Kit

products full name :

Human MGST1 (Microsomal Glutathione S Transferase 1) ELISA Kit

products short name :

[MGST1]

other names :

[microsomal glutathione S-transferase 1 isoform c; Microsomal glutathione S-transferase 1; microsomal glutathione S-transferase 1; glutathione S-transferase 12; microsomal GST-1; microsomal GST-I; microsomal glutathione S-transferase 1; Microsomal GST-I]

products gene name :

[MGST1]

other gene names :

[MGST1; MGST1; MGST; GST12; MGST-I; GST12; MGST; Microsomal GST-1]

uniprot entry name :

MGST1_HUMAN

reactivity :

Human

sequence length :

specificity :

This kit recognizes natural and recombinant Human MGST1. No significant cross-reactivity or interference between Human MGST1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100mIU/mL. Sensitivity: Min: 0.938mIU/mL; Max: 100mIU/mL

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MGST1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MGST1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MGST1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MGST1. You can calculate the concentration of MGST1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

390125204

ncbi acc num :

NP_001254527.1

ncbi gb acc num :

NM_001267598.1

uniprot acc num :

P10620

ncbi mol weight :

9,984 Da

ncbi pathways :

Aflatoxin Activation And Detoxification Pathway (1127511); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective ACY1 Causes Encephalopathy Pathway (1127638); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663)

ncbi summary :

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]

uniprot summary :

MGST1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. Belongs to the MAPEG family. Protein type: Other Amino Acids Metabolism - glutathione; Membrane protein, integral; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Membrane protein, multi-pass; Mitochondrial; EC 2.5.1.18; Transferase. Chromosomal Location of Human Ortholog: 12p12.3-p12.1. Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; apical part of cell; mitochondrial inner membrane; integral to membrane; nucleus. Molecular Function: protein binding; protein homodimerization activity; glutathione transferase activity; glutathione binding; glutathione peroxidase activity. Biological Process: response to drug; Leydig cell differentiation; glutathione metabolic process; xenobiotic metabolic process; response to lipopolysaccharide; response to organic nitrogen

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590