Rat C5a (Complement Component 5a) ELISA Kit | MyBioSource MBS2506864 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat C5a (Complement Component 5a) ELISA Kit

catalog :

MBS2506864

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Lee S, Hwang S, Jeong J, Han Y, Kim S, Lee D, et al. Nickel oxide nanoparticles can recruit eosinophils in the lungs of rats by the direct release of intracellular eotaxin. Part Fibre Toxicol. 2016;13:30 pubmed publisher

product information

catalog number :

MBS2506864

products type :

ELISA Kit

products full name :

Rat C5a (Complement Component 5a) ELISA Kit

products short name :

C5a

other names :

C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a-R; complement component 5 receptor 1; complement component 5a receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD_antigen: CD88

products gene name :

C5a

other gene names :

C5AR1; C5AR1; C5A; C5AR; C5R1; CD88; C5AR; C5R1; C5a-R; C5aR

uniprot entry name :

C5AR1_HUMAN

reactivity :

Rat

sequence length :

350

specificity :

This kit recognizes natural and recombinant Rat C5a. No significant cross-reactivity or interference between Rat C5a and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: The minimum detectable dose of Rat C5a is 37.5pg/mL

products description :

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Rat C5a concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C5a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C5a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C5a. You can calculate the concentration of C5a in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502509

ncbi acc num :

NP_001727.1

ncbi gb acc num :

NM_001736.3

uniprot acc num :

P21730

ncbi mol weight :

39,336 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

uniprot summary :

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia. Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: cell surface; integral to plasma membrane; apical part of cell; basolateral plasma membrane; cytoplasmic membrane-bound vesicle; plasma membrane. Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity. Biological Process: neutrophil chemotaxis; sensory perception of chemical stimulus; organ regeneration; activation of MAPK activity; apoptosis; response to lipopolysaccharide; chemotaxis; signal transduction; cell proliferation in hindbrain; mRNA transcription from RNA polymerase II promoter; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage