ELISA/assay

Human PRCP (Prolylcarboxypeptidase) ELISA Kit

MBS2506854

48-Strip-Wells

410 USD

ELISA Kit

Human PRCP (Prolylcarboxypeptidase) ELISA Kit

PRCP

Prcp protein; Lysosomal Pro-X carboxypeptidase; lysosomal Pro-X carboxypeptidase; proline carboxypeptidase; prolylcarboxypeptidase (angiotensinase C); Proline carboxypeptidase; Prolylcarboxypeptidase; PRCP

PRCP

Prcp; Prcp; PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik; PRCP

PCP_MOUSE

Human

491

This kit recognizes natural and recombinant Human PRCP. No significant cross-reactivity or interference between Human PRCP and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human PRCP is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PRCP concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRCP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRCP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PRCP. You can calculate the concentration of PRCP in the samples by comparing the OD of the samples to the standard curve.

32967631

AAH55022.1

Q7TMR0

11,803 Da

Formation Of Fibrin Clot (Clotting Cascade) Pathway (1110054); Hemostasis Pathway (1110026); Intrinsic Pathway (1110056); Protein Digestion And Absorption Pathway (172853); Protein Digestion And Absorption Pathway (171868)

PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family. Protein type: EC 3.4.16.2; Protease. Cellular Component: lysosome. Molecular Function: peptidase activity; carboxypeptidase activity; hydrolase activity; serine-type peptidase activity. Biological Process: plasma kallikrein-kinin cascade; negative regulation of systemic arterial blood pressure; angiogenesis involved in wound healing; glucose homeostasis; regulation of blood vessel endothelial cell migration; proteolysis

48-Strip-Wells

410 USD

96-Strip-Wells

490