catalog number :
MBS2506843
products type :
ELISA Kit
products full name :
Rat IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1) ELISA Kit
products short name :
[IGFBP-1]
other names :
[insulin-like growth factor-binding protein 1; Insulin-like growth factor-binding protein 1; insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; placental protein 12; insulin-like growth factor binding protein 1; Placental protein 12; PP12]
products gene name :
[IGFBP-1]
other gene names :
[IGFBP1; IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12]
uniprot entry name :
IBP1_HUMAN
specificity :
This kit recognizes Rat IgE in samples. No significant cross-reactivity or interference between Rat IgE and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IgE concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IgE. You can calculate the concentration of Rat IgE in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_000587.1
ncbi gb acc num :
NM_000596.2
ncbi mol weight :
27,904 Da
ncbi pathways :
ATF4 Activates Genes Pathway (530772); AhR Pathway (755436); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Of Proteins Pathway (106230); Myometrial Relaxation And Contraction Pathways (198759); PERK Regulates Gene Expression Pathway (105907); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908); Unfolded Protein Response (UPR) Pathway (105904)
ncbi summary :
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
uniprot summary :
IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7p12.3. Cellular Component: extracellular space; extracellular region. Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding. Biological Process: cellular protein metabolic process; regulation of insulin-like growth factor receptor signaling pathway; unfolded protein response, activation of signaling protein activity; unfolded protein response; tissue regeneration; insulin receptor signaling pathway; signal transduction; positive regulation of cell growth
size5 :
10x96-Strip-Wells