ELISA/assay

Rat DPD (Deoxypyridinoline) ELISA Kit

MBS2506789

48-Strip-Wells

410 USD

ELISA Kit

Rat DPD (Deoxypyridinoline) ELISA Kit

DPD

dihydropyrimidine dehydrogenase; Dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase; dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase

DPD

Dpyd; Dpyd; DPD; DHPDHase; DPD

DPYD_RAT

Rat

1025

This kit recognizes natural and recombinant Rat DPD. No significant cross-reactivity or interference between Rat DPD and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 3.125-200ng/mL. Sensitivity: The minimum detectable dose of Rat DPD is 1.875ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat DPD concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DPD. During the reaction, DPD in the sample or standard competes with a fixed amount of DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPD in the samples is then determined by comparing the OD of the samples to the standard curve.

13591940

NP_112289.1

NM_031027.1

O89000

111,468 Da

Drug Metabolism - Other Enzymes Pathway (83425); Drug Metabolism - Other Enzymes Pathway (428); Metabolic Pathways (132933); Pantothenate And CoA Biosynthesis Pathway (83407); Pantothenate And CoA Biosynthesis Pathway (401); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (606001); Pyrimidine Degradation, Uracil = Beta-alanine, Thymine = 3-aminoisobutanoate Pathway (468239); Pyrimidine Metabolism Pathway (83339); Pyrimidine Metabolism Pathway (309); Beta-Alanine Metabolism Pathway (83358)

catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family. Protein type: Nucleotide Metabolism - pyrimidine; Other Amino Acids Metabolism - beta-alanine; EC 1.3.1.2; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes. Cellular Component: cytoplasm; cytosol. Molecular Function: protein homodimerization activity; uracil binding; 4 iron, 4 sulfur cluster binding; dihydroorotate dehydrogenase activity; metal ion binding; iron-sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding. Biological Process: uracil metabolic process; response to drug; circadian rhythm; uracil catabolic process; UMP biosynthetic process; response to glucocorticoid stimulus; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; response to nutrient; pyrimidine base catabolic process; thymidine catabolic process

48-Strip-Wells

410 USD

96-Strip-Wells

490