Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit | MyBioSource MBS2506773 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit

catalog :

MBS2506773

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2506773

products type :

ELISA Kit

products full name :

Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit

products short name :

[BMP-2]

other names :

[bone morphogenetic protein 2; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A]

products gene name :

[BMP-2]

other gene names :

[BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A]

uniprot entry name :

BMP2_HUMAN

reactivity :

Human

sequence length :

396

specificity :

This kit recognizes Human BMG/beta2-MG in samples. No significant cross-reactivity or interference between Human BMG/beta2-MG and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Urine. Please Consult Technical Support For The Applicability If Other Biological Fluids Need To Be Tested. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BMG/beta2-MG were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BMG/beta2-MG were tested on 3 different plates,20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human BMG/beta2-MG concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BMG/beta2-MG. Samples (or Standards) and biotinylated detection antibody specific for Human BMG/beta2-MG are added to the micro ELISA plate wells. Human BMG/beta2-MG would combined with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BMG/beta2-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human BMG/beta2-MG. You can calculate the concentration of Human BMG/beta2-MG in the samples by comparing the OD of the samples to the standard curve!!Background/Introduction: Beta 2 microglobulin (beta2M) is a small protein (11,800 Dalton), normally found on the surface of many cells, including lymphocytes, and in small amounts in the blood and urine [1]. An increased amount in the blood or urine may be a sign of certain diseases, including some types of cancer, such as multiple myeloma or lymphoma.beta2M is critical for the immune surveillance and modulation in vertebrate animals. The dysregulation of beta2M is associated with multiple diseases, including endogenous and infectious diseases. beta2M could directly participate in the development of cancer cells, and the level of beta2M is deemed as a prognostic marker for several malignancies. It also involves in forming major histocompatibility complex (MHC class I or MHC I) or like heterodimers, covering from antigen presentation to immune homeostasis [2].

ncbi gi num :

6649952

ncbi acc num :

AAF21646.1

uniprot acc num :

P12643

ncbi mol weight :

44,702 Da

ncbi pathways :

Adipogenesis Pathway (198832); BDNF Signaling Pathway (712093); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); BMP Signalling And Regulation Pathway (198910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (730310)

ncbi summary :

The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]

uniprot summary :

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805