ELISA/assay

Human IFN-alpha (Interferon Alpha) ELISA Kit

MBS2506739

96-Strip-Wells

445 USD

ELISA Kit

Human IFN-alpha (Interferon Alpha) ELISA Kit

IFN-alpha

interferon alpha-1/13; Interferon alpha-1/13; interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D; interferon, alpha 1; Interferon alpha-D; LeIF D

IFN-alpha

IFNA1; IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD; IFN-alpha-1/13; LeIF D

IFNA1_HUMAN

Human

189

This kit recognizes natural and recombinant Human IFN-alpha. No significant cross-reactivity or interference between Human IFN-alpha and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 9.375pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IFN-alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-alpha. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IFN-alpha. You can calculate the concentration of IFN-alpha in the samples by comparing the OD of the samples to the standard curve.

13128950

NP_076918.1

NM_024013.2

P01562

21,725 Da

Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)

The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]

IFNA1/13: a cytokine that belongs to the alpha/beta interferon family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response

96-Strip-Wells

445 USD