Porcine TH (Tyrosine Hydroxylase) ELISA Kit | MyBioSource MBS2506643 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine TH (Tyrosine Hydroxylase) ELISA Kit

catalog :

MBS2506643

quantity :

48-Strip-Wells

price :

450 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2506643

products type :

ELISA Kit

products full name :

Porcine TH (Tyrosine Hydroxylase) ELISA Kit

products short name :

other names :

tyrosine 3-monooxygenase isoform a; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH

products gene name :

other gene names :

TH; TH; TYH; DYT14; DYT5b; TYH; TH

uniprot entry name :

TY3H_HUMAN

reactivity :

Porcine

sequence length :

528

specificity :

This kit recognizes natural and recombinant Porcine TH. No significant cross-reactivity or interference between Porcine TH and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine TH concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TH. You can calculate the concentration of TH in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

88900501

ncbi acc num :

NP_954986.2

ncbi gb acc num :

NM_199292.2

uniprot acc num :

P07101

ncbi mol weight :

44,898 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alpha-synuclein Signaling Pathway (137913); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357)

ncbi summary :

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: Oxidoreductase; Vesicle; Endoplasmic reticulum; Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Mitochondrial. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; melanosome membrane; dendrite; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus. Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity. Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; synaptic transmission, dopaminergic; response to peptide hormone stimulus; locomotory behavior; fatty acid metabolic process; response to salt stress; regulation of heart contraction; response to estradiol stimulus; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis. Disease: Segawa Syndrome, Autosomal Recessive

size1 :

48-Strip-Wells

price1 :

450 USD

size2 :

96-Strip-Wells

price2 :

535

more info or order :

MyBioSource product webpage