catalog number :
MBS2506610
products type :
ELISA Kit
products full name :
Human PIIICP (Procollagen III C-Terminal ProPeptide) ELISA Kit
products short name :
PIIICP
products gene name :
PIIICP
specificity :
This assay has high sensitivity and excellent specificity for detection of Human PIIICP. No significant cross-reactivity or interference between Human PIIICP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PIIICP and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 12.5--800pg/mL. Sensitivity: 7.5pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIIICP in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIIICP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PIIICP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIIICP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PIIICP. You can calculate the concentration of Human PIIICP in the samples by comparing the OD of the samples to the standard curve.