catalog number :
MBS2506564
products type :
ELISA Kit
products full name :
Guinea pig IFN-alpha (Interferon Alpha) ELISA Kit
products short name :
IFN-alpha
other names :
interferon alpha-1/13; Interferon alpha-1/13; interferon alpha-1/13; IFN-alpha 1b; IFN-alpha-1/13; interferon alpha 1b; interferon alpha-D; interferon-alpha1; leIF D; interferon, alpha 1; Interferon alpha-D; LeIF D
products gene name :
IFN-alpha
other gene names :
IFNA1; IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD; IFN-alpha-1/13; LeIF D
uniprot entry name :
IFNA1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Guinea pig IFN-alpha. No significant cross-reactivity or interference between Guinea pig IFN-alpha and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Guinea pig IFN-alpha and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625--1000pg/mL. Sensitivity: 9.375pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFN-alpha in Guinea pig serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IFN-alpha. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Guinea pig IFN-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pig IFN-alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Guinea pig IFN-alpha. You can calculate the concentration of Guinea pig IFN-alpha in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_076918.1
ncbi gb acc num :
NM_024013.2
ncbi mol weight :
21,725 Da
ncbi pathways :
Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)
ncbi summary :
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
uniprot summary :
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation during immune response; innate immune response; blood coagulation; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response