Porcine NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | MyBioSource MBS2506548 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

catalog :

MBS2506548

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2506548

products type :

ELISA Kit

products full name :

Porcine NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

products short name :

[NGAL]

other names :

[neutrophil gelatinase-associated lipocalin; Neutrophil gelatinase-associated lipocalin; neutrophil gelatinase-associated lipocalin; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; lipocalin-2; migration-stimulating factor inhibitor; oncogene 24p3; p25; siderocalin LCN2; lipocalin 2; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25]

products gene name :

[NGAL]

other gene names :

[LCN2; LCN2; 24p3; MSFI; NGAL; HNL; NGAL; NGAL]

uniprot entry name :

NGAL_HUMAN

reactivity :

Porcine

sequence length :

198

specificity :

This kit recognizes Porcine MYBPC3 in samples. No significant cross-reactivity or interference between PorcineMYBPC3and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.09ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Porcine MYBPC3 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Porcine MYBPC3 were testedon 3 different plates, 20 replicates in eachplate

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine MYBPC3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porc ine MYBPC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine MYBPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine MYBPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PorcineMYBPC3. You can calculate the concentration of Porcine MYBPC3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

38455402

ncbi acc num :

NP_005555.2

ncbi gb acc num :

NM_005564.3

uniprot acc num :

P80188

ncbi mol weight :

22,457 Da

ncbi pathways :

Iron Uptake And Transport Pathway (187191); Orphan Transporters Pathway (1127546); Transmembrane Transport Of Small Molecules Pathway (106572)

uniprot summary :

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q34. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding. Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975