Rat LBP (Lipolysaccharide Binding Protein) ELISA Kit | MyBioSource MBS2506538 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat LBP (Lipolysaccharide Binding Protein) ELISA Kit

catalog :

MBS2506538

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2506538

products type :

ELISA Kit

products full name :

Rat LBP (Lipolysaccharide Binding Protein) ELISA Kit

products short name :

[LBP]

other names :

[lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; BPI fold containing family D, member 2; LPS-binding protein; lipopolysaccharide binding protein]

products gene name :

[LBP]

other gene names :

[LBP; LBP; BPIFD2; LBP]

uniprot entry name :

LBP_HUMAN

reactivity :

Rat

sequence length :

481

specificity :

This kit recognizes natural and recombinantRatLBP. No significant cross-reactivity or interference between RatLBP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.875-120ng/mL. Sensitivity: Min: 1.125ng/mL; Max: 120ng/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatLBPconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LBP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LBPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LBP.You can calculate the concentration of LBPin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

31652249

ncbi acc num :

NP_004130.2

ncbi gb acc num :

NM_004139.4

uniprot acc num :

P18428

ncbi mol weight :

53,384 Da

ncbi pathways :

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388); Toll-like Receptor Signaling Pathway (83076)

ncbi summary :

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

uniprot summary :

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; lipopolysaccharide binding; receptor binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; toll-like receptor signaling pathway; cellular defense response; innate immune response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590