Human MASP2 (Mannan Associated Serine Protease 2) ELISA Kit | MyBioSource MBS2506522 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MASP2 (Mannan Associated Serine Protease 2) ELISA Kit

catalog :

MBS2506522

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2506522

products type :

ELISA Kit

products full name :

Human MASP2 (Mannan Associated Serine Protease 2) ELISA Kit

products short name :

[MASP2]

other names :

[mannan-binding lectin serine protease 2 isoform 1 preproprotein; Mannan-binding lectin serine protease 2; mannan-binding lectin serine protease 2; MBL-associated plasma protein of 19 kD; MBL-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; small MBL-associated protein; mannan-binding lectin serine peptidase 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2]

products gene name :

[MASP2]

other gene names :

[MASP2; MASP2; sMAP; MAP19; MASP-2; MASP1P1; MASP-2]

uniprot entry name :

MASP2_HUMAN

reactivity :

Human

sequence length :

686

specificity :

This kit recognizes Human MAPtau in samples. No significant cross-reactivity or interference between HumanMAPtau and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: 4.69pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision withinan assay): 3 samples with low, midrange and high level HumanMAPtauwere tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanMAPtau were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MAPtau concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAPtau. St andards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAPtau and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAPtau, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MAPtau. You can calculate the concentration of Human MAPtau in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

21264363

ncbi acc num :

NP_006601.2

ncbi gb acc num :

NM_006610.3

uniprot acc num :

O00187

ncbi mol weight :

20,629 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); Ficolins Bind To Repetitive Carbohydrate Structures On The Target Cell Surface Pathway (833818); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Lectin Pathway Of Complement Activation (106408)

ncbi summary :

The Ra-reactive factor (RARF) is a complement-dependent bactericidal factor that binds to the Ra and R2 polysaccharides expressed by certain enterobacteria. Alternate splicing of this gene results in two transcript variants encoding two RARF components that are involved in the mannan-binding lectin pathway of complement activation. The longer isoform is cleaved into two chains which form a heterodimer linked by a disulfide bond. The encoded proteins are members of the trypsin family of peptidases. [provided by RefSeq, Jul 2008]

uniprot summary :

MASP2: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. Defects in MASP2 are the cause of MASP2 deficiency (MASPD). MASPD is a disorder that results in autoimmune manifestations, recurrent severe infections, and chronic inflammatory disease. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.104; Protease; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1p36.3-p36.2. Cellular Component: extracellular region. Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; complement component C4b binding; calcium ion binding; calcium-dependent protein binding. Biological Process: innate immune response; complement activation, lectin pathway; proteolysis; complement activation, classical pathway; complement activation. Disease: Masp2 Deficiency

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage