ELISA/assay

Rat PINP (Procollagen Type I N-Terminal Propeptide) ELISA Kit

MBS2506450

48-Strip-Wells

410 USD

ELISA Kit

Rat PINP (Procollagen Type I N-Terminal Propeptide) ELISA Kit

PINP

PINP

Rat

This kit recognizes natural and recombinant Rat PINP. No significant cross-reactivity or interference between Rat PINP and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PINP concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PINP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PINP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PINP. You can calculate the concentration of PINP in the samples by comparing the OD of the samples to the standard curve.

48-Strip-Wells

410 USD

96-Strip-Wells

490