ELISA/assay

Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit

MBS2506440

48-Strip-Wells

410 USD

ELISA Kit

Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit

TFF3

trefoil factor 3; Trefoil factor 3; trefoil factor 3; polypeptide P1.B; trefoil factor 3 (intestinal); Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B

TFF3

TFF3; TFF3; ITF; P1B; TFI; ITF; TFI; hITF; hP1.B

TFF3_HUMAN

Human

94

This assay has high sensitivity and excellent specificity for detection of Human TFF3. No significant cross-reactivity or interference between Human TFF3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human TFF3 and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125--5000pg/mL. Sensitivity: 46.875pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TFF3 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFF3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TFF3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFF3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TFF3. You can calculate the concentration of Human TFF3 in the samples by comparing the OD of the samples to the standard curve.

281485608

NP_003217.3

NM_003226.3

Q07654

8,641 Da

HIF-1-alpha Transcription Factor Network Pathway (138045); IL4-mediated Signaling Events Pathway (137933)

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule. Biological Process: response to peptide hormone stimulus; digestion; defense response

48-Strip-Wells

410 USD

96-Strip-Wells

490