ELISA/assay

Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit

MBS2506383

24-Strip-Wells

215 USD

ELISA Kit

Human ACE2 (Angiotensin I Converting Enzyme 2) ELISA Kit

[ACE2]

[angiotensin-converting enzyme 2; Angiotensin-converting enzyme 2; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme homolog; metalloprotease MPROT15; angiotensin I converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15Processed angiotensin-converting enzyme 2]

[ACE2]

[ACE2; ACE2; ACEH; ACEH]

ACE2_HUMAN

Human

805

This kit recognizes Human, Monkey, Mouse E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Saliva, Urine Samples. Assay Type: Quantitative Competitive. Detection Range: 9.37-600pg/mL. Sensitivity: 5.63pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 human samples with low, mid range and high level E2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 human samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human, Monkey, Mouse E2 concentrations in serum, plasma, saliva, urine samples. Please contact tech-support for other sample type detection. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve!!Background/Introduction: Estradiol is a female sex hormone produced by the ovaries, adrenal gland and also the placenta during pregnancy. Estradiol is the most important hormone during a female's reproductive years, and is required for reproductive and sexual function as well as having an impact on the health of other organs and tissues. In females, estradiol acts primarily as a growth hormone for the reproductive organs including the vagina, the fallopian tubes, the endometrium and the cervical glands [1]. Estradiol also enhances growth of the womb's muscle layer, the myometrium. In addition, the hormone maintains oocytes and triggers a series of events that lead to ovulation. Though estradiol levels in men are much lower compared to those in women, estradiol has important roles in men as well. Aside from humans and other mammals, estradiol is also found in most vertebrates and crustaceans, insects, fish, and other animal species. Estradiol is produced especially within the follicles of the ovaries, but also in other tissues including the testicles, the adrenal glands, fat, liver, the breasts, and the brain. Estradiol is produced in the body from cholesterol through a series of reactions and intermediates [2].

11225609

NP_068576.1

NM_021804.2

Q9BYF1

63,912 Da

ACE Inhibitor Pathway (198763); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Protease; EC 3.4.17.23. Chromosomal Location of Human Ortholog: Xp22. Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; extracellular region; plasma membrane; lipid raft. Molecular Function: viral receptor activity; peptidyl-dipeptidase activity; protein binding; metallopeptidase activity; carboxypeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding. Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; angiotensin catabolic process in blood; regulation of inflammatory response; receptor biosynthetic process; angiotensin mediated drinking behavior

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590