catalog number :
MBS2506194
products type :
ELISA Kit
products full name :
Porcine IL-8 (Interleukin 8) ELISA Kit
products short name :
[IL-8]
other names :
[interleukin-8; Interleukin-8; interleukin-8; C-X-C motif chemokine 8; IL-8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; chemokine (C-X-C motif) ligand 8; Alveolar macrophage chemotactic factor I; AMCF-I; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8]
products gene name :
[IL-8]
other gene names :
[IL8; CXCL8; CXCL8; AMCF-I; IL8; IL-8; AMCF-I]
uniprot entry name :
IL8_PIG
specificity :
This kit recognizes Porcine IL-2 in samples. No significant cross-reactivity or interference between Porcine IL-2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine IL-2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine IL-2 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine IL-2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine IL-2. You can calculate the concentration of Porcine IL-2 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_999032.1
ncbi gb acc num :
NM_213867.1
ncbi mol weight :
11,633 Da
ncbi pathways :
Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Cellular Senescence Pathway (1125456); Cellular Responses To Stress Pathway (1125444); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1124657); Chemokine Signaling Pathway (98758)
uniprot summary :
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
size5 :
10x96-Strip-Wells