Human P-GP (Permeability Glycoprotein) ELISA Kit | MyBioSource MBS2506188 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human P-GP (Permeability Glycoprotein) ELISA Kit

catalog :

MBS2506188

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2506188

products type :

ELISA Kit

products full name :

Human P-GP (Permeability Glycoprotein) ELISA Kit

products short name :

[P-GP]

other names :

[P-glycoprotein; ATP-binding cassette sub-family B member 5; ATP-binding cassette sub-family B member 5; ABCB5 P-gp; ATP-binding cassette protein; P-glycoprotein ABCB5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; ABCB5 P-gp; P-glycoprotein ABCB5]

products gene name :

[P-GP]

other gene names :

[ABCB5; ABCB5; ABCB5beta; EST422562; ABCB5alpha]

uniprot entry name :

ABCB5_HUMAN

reactivity :

Human

sequence length :

832

specificity :

This kit recognizes Human PF4V1 in samples. No Significant cross-reactivity or interference between Human PF4V1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100 ng/mL. Sensitivity: 0.94 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PF4V1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PF4V1 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PF4V1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PF4V1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PF4V1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PF4V1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PF4V1. You can calculate the concentration of Human PF4V1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

33307712

ncbi acc num :

AAQ03033.1

uniprot acc num :

Q2M3G0

ncbi mol weight :

89,832 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (106573); Transmembrane Transport Of Small Molecules Pathway (106572)

ncbi summary :

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

uniprot summary :

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter, ABC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7p21.1. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding. Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage