Chicken IgM (Immunoglobulin M) ELISA Kit | MyBioSource MBS2506018 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken IgM (Immunoglobulin M) ELISA Kit

catalog :

MBS2506018

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2506018

products type :

ELISA Kit

products full name :

Chicken IgM (Immunoglobulin M) ELISA Kit

products short name :

[IgM]

other names :

[ubiquitin-conjugating enzyme E2 A isoform 4; Ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; ubiquitin carrier protein A; ubiquitin-protein ligase A; ubiquitin-conjugating enzyme E2A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A]

products gene name :

[IgM]

other gene names :

[UBE2A; UBE2A; UBC2; HHR6A; MRXSN; RAD6A; MRXS30; RAD6A; HR6A; hHR6A]

uniprot entry name :

UBE2A_HUMAN

reactivity :

Chicken

sequence length :

119

specificity :

This kit recognizes ChickenIgAin samples. No significantcross-reactivity or interference between ChickenIgA and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ChickenIgA were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ChickenIgA were testedon 3 different plates, 20 replicates in eachplate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of ChickenIgA concentrations in ser um, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ChickenIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ChickenIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ChickenIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ChickenIgA. You can calculate the concentration of ChickenIgA in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

531990811

ncbi acc num :

NP_001269090.1

ncbi gb acc num :

NM_001282161.1

uniprot acc num :

P49459

ncbi mol weight :

8,833 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

uniprot summary :

UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA. Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase. Chromosomal Location of Human Ortholog: Xq24. Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol. Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; maternal process involved in pregnancy; protein polyubiquitination; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV. Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975

more info or order :

MyBioSource product webpage