catalog number :
MBS2506005
products type :
ELISA Kit
products full name :
Human VASPIN (Visceral Adipose Specific Serine Protease Inhibitor) ELISA Kit
products short name :
[VASPIN]
other names :
[visceral adipose tissue specific SERPIN; Serpin A12; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin]
products gene name :
[VASPIN]
other gene names :
[Serpina12; Vaspin]
uniprot entry name :
SPA12_RAT
specificity :
This kit recognizes Human UCHL1 in samples. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Human UCHL1 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Human UCHL1 were testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human UCHL1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human UCHL1. You can calculate the concentration of Human UCHL1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAL99574.1
ncbi mol weight :
47,527 Da
uniprot summary :
SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family. Protein type: Inhibitor; Secreted; Secreted, signal peptide. Cellular Component: extracellular space; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; glucose metabolic process; positive regulation of insulin receptor signaling pathway
size5 :
10x96-Strip-Wells