Human CFL1(Cofilin 1, Non-Muscle) ELISA Kit | MyBioSource MBS2505977 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CFL1(Cofilin 1, Non-Muscle) ELISA Kit

catalog :

MBS2505977

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505977

products type :

ELISA Kit

products full name :

Human CFL1(Cofilin 1, Non-Muscle) ELISA Kit

products short name :

[CFL1]

other names :

[cofilin; Cofilin-1; cofilin-1; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15; p18; cofilin 1 (non-muscle); 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform]

products gene name :

[CFL1]

other gene names :

[CFL1; CFL1; CFL; HEL-S-15; CFL; p18]

uniprot entry name :

COF1_HUMAN

reactivity :

Human

sequence length :

166

specificity :

This kit recognizes Human CD34 in samples. No Significant cross-reactivity or interference between Human CD34 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10 ng/mL. Sensitivity: 0.10 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CD34 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CD34 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CD34 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD34. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD34, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD34. You can calculate the concentration of Human CD34 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

1177471

ncbi acc num :

CAA64685.1

uniprot acc num :

P23528

ncbi mol weight :

18,502 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); CXCR4-mediated Signaling Events Pathway (137910); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691); EPHB-mediated Forward Signaling Pathway (1127693); Fc Gamma R-mediated Phagocytosis Pathway (114228)

ncbi summary :

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

uniprot summary :

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle. Molecular Function: protein binding; actin binding. Biological Process: axon guidance; platelet activation; actin filament depolymerization; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590