ELISA/assay

Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit

MBS2505942

96-Strip-Wells

445 USD

ELISA Kit

Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible)ELISA Kit

NOS2/iNOS

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOS type II; NOS, type II; hepatocyte NOS; inducible NO synthase; inducible NOS; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

NOS2/iNOS

NOS2; NOS2; NOS; INOS; NOS2A; HEP-NOS; NOS2A; HEP-NOS; Inducible NOS; iNOS

NOS2_HUMAN

Rat

1153

This kit recognizes natural and recombinant Rat NOS2/iNOS. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of Rat NOS2/iNOS is 0.188ng/mL

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NOS2/iNOS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NOS2/iNOS. You can calculate the concentration of NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

24041029

NP_000616.3

NM_000625.4

P35228

126,749 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795)

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; cytosol; nucleus. Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; NADP binding; receptor binding. Biological Process: circadian rhythm; interaction with host; superoxide metabolic process; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; defense response to Gram-negative bacterium; positive regulation of vasodilation; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; negative regulation of blood pressure; inflammatory response; nitric oxide biosynthetic process; negative regulation of protein catabolic process; blood coagulation; regulation of insulin secretion; nitric oxide mediated signal transduction. Disease: Hypertension, Essential; Malaria, Susceptibility To

96-Strip-Wells

445 USD