Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit | MyBioSource MBS2505910 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit

catalog :

MBS2505910

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2505910

products type :

ELISA Kit

products full name :

Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit

products short name :

PADI4

other names :

protein-arginine deiminase type-4; Protein-arginine deiminase type-4; protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase, type V; protein-arginine deiminase type IV; peptidyl arginine deiminase, type IV; HL-60 PAD; Peptidylarginine deiminase IV; Protein-arginine deiminase type IV

products gene name :

PADI4

other gene names :

PADI4; PADI4; PAD; PAD4; PDI4; PDI5; PADI5; PADI5; PDI5

uniprot entry name :

PADI4_HUMAN

reactivity :

Human

sequence length :

663

specificity :

This kit recognizes natural and recombinant Human PADI4. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.188-12ng/mL. Sensitivity: 0.113ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PADI4 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PADI4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PADI4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PADI4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PADI4. You can calculate the concentration of PADI4 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

216548487

ncbi acc num :

NP_036519.2

ncbi gb acc num :

NM_012387.2

uniprot acc num :

Q9UM07

ncbi mol weight :

74,079 Da

ncbi pathways :

Protein Citrullination Pathway (142167); Protein Citrullination Pathway (138213)

ncbi summary :

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

uniprot summary :

PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at Arg-8 and/or Arg-17 and histone H4 at Arg-3 , which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at Arg-2142 , which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family. Protein type: Nuclear receptor co-regulator; EC 3.5.3.15; Hydrolase. Chromosomal Location of Human Ortholog: 1p36.13. Cellular Component: cytoplasm; nucleus. Molecular Function: arginine deiminase activity; protein binding; calcium ion binding; protein-arginine deiminase activity. Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; protein modification process; innate immune response; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine. Disease: Rheumatoid Arthritis

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage