catalog number :
MBS2505798
products type :
ELISA Kit
products full name :
Human ACVA (Activin A) ELISA Kit
products short name :
[ACVA]
other names :
[acv synthetase, partial; Acv synthetase; Acv synthetase]
products gene name :
[ACVA]
other gene names :
[acvA]
uniprot entry name :
Q4J6A7_EMEND
specificity :
This kit recognizes Human ACTH in samples. No significant cross-reactivity or interference between Human ACTH and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ACTH were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ACTH were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ACTH concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ACTH. During the reaction, Human ACTH in the sample or standard competes with a fixed amount of Human ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ACTH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAA03916.2
ncbi mol weight :
69,529 Da
size5 :
10x96-Strip-Wells