catalog number :
MBS2505792
products type :
ELISA Kit
products full name :
Rat GUSb (Glucuronidase, Beta) ELISA Kit
products short name :
[GUSb]
other names :
[GUSB protein; Beta-glucuronidase; beta-glucuronidase; glucuronidase, beta<]
products gene name :
[GUSb]
other gene names :
[GUSB; GUSB]
uniprot entry name :
A3KMY8_BOVIN
specificity :
This kit recognizes natural and recombinant Rat GUSb. No significant cross-reactivity or interference between Rat GUSb and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GUSb concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GUSb. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GUSb and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GUSb, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GUSb. You can calculate the concentration of GUSb in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI33416.1
ncbi mol weight :
74,470 Da
ncbi pathways :
Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (391064); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (468309); Chondroitin Sulfate Degradation Pathway (391009); Chondroitin Sulfate Degradation Pathway (468247); Defective B3GAT3 Causes JDSSDHD Pathway (1119862); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1119866); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1119861); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1119865); Defective CHST3 Causes SEDCJD Pathway (1119864); Defective CHST6 Causes MCDC1 Pathway (1119867)
uniprot summary :
Plays an important role in the degradation of dermatan and keratan sulfates.
size5 :
10x96-Strip-Wells