catalog number :
MBS2505782
products type :
ELISA Kit
products full name :
Human MCAM (Melanoma Cell Adhesion Molecule) ELISA Kit
products short name :
MCAM
other names :
Mcam protein; Mcam protein; cell surface glycoprotein MUC18; cell adhesion molecule; melanoma cell adhesion molecule b; Mcam protein
products gene name :
MCAM
other gene names :
mcamb; mcamb; mcam; cd146; gicerin; id:ibd3512
uniprot entry name :
B3DH03_DANRE
specificity :
This assay has high sensitivity and excellent specificity for detection of Human MCAM. No significant cross-reactivity or interference between Human MCAM and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human MCAM and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.625--40ng/mL. Sensitivity: 0.375ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCAM in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCAM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human MCAM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MCAM. You can calculate the concentration of Human MCAM in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI62589.1
ncbi mol weight :
69,093 Da