Chicken EPO (Erythropoietin) ELISA Kit | MyBioSource MBS2505778 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken EPO (Erythropoietin) ELISA Kit

catalog :

MBS2505778

quantity :

48-Strip-Wells

price :

400 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2505778

products type :

ELISA Kit

products full name :

Chicken EPO (Erythropoietin) ELISA Kit

products short name :

EPO

other names :

erythropoietin; Erythropoietin; erythropoietin; epoetin; erythropoietin; INN: Epoetin

products gene name :

EPO

other gene names :

EPO; EPO; EP; MVCD2

uniprot entry name :

EPO_HUMAN

reactivity :

Chicken

sequence length :

193

specificity :

This kit recognizes natural and recombinant Chicken EPO. No significant cross-reactivity or interference between Chicken EPO and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken EPO concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EPO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for EPO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of EPO. You can calculate the concentration of EPO in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

62240997

ncbi acc num :

NP_000790.2

ncbi gb acc num :

NM_000799.2

uniprot acc num :

P01588

ncbi mol weight :

21,307 Da

ncbi pathways :

Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EPO Receptor Signaling Pathway (198882); EPO Signaling Pathway (138051); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]

uniprot summary :

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 7q22. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; erythropoietin receptor binding; hormone activity; protein kinase activator activity. Biological Process: hemoglobin biosynthetic process; erythrocyte maturation; blood circulation; apoptosis; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to testosterone stimulus; signal transduction; response to salt stress; response to vitamin A; peptidyl-serine phosphorylation; response to hyperoxia; positive regulation of tyrosine phosphorylation of Stat5 protein; response to estrogen stimulus; positive regulation of protein kinase activity; positive regulation of cell proliferation; response to axon injury; positive regulation of Ras protein signal transduction; response to electrical stimulus; positive regulation of neuron differentiation; embryo implantation; positive regulation of DNA replication; aging. Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

size1 :

48-Strip-Wells

price1 :

400 USD

size2 :

96-Strip-Wells

price2 :

475

more info or order :

MyBioSource product webpage