Mouse LTF/LF (Lactoferrin) ELISA Kit | MyBioSource MBS2505730 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse LTF/LF (Lactoferrin) ELISA Kit

catalog :

MBS2505730

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2505730

products type :

ELISA Kit

products full name :

Mouse LTF/LF (Lactoferrin) ELISA Kit

products short name :

[LTF/LF]

other names :

[lactotransferrin isoform 1; Lactotransferrin; lactotransferrin; epididymis luminal protein 110; growth-inhibiting protein 12; kaliocin-1; lactoferricin; lactoferroxin; neutrophil lactoferrin; talalactoferrin; lactotransferrin; Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C]

products gene name :

[LF/LTF]

other gene names :

[LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H]

uniprot entry name :

TRFL_HUMAN

reactivity :

Mouse

sequence length :

710

specificity :

This kit recognizes natural and recombinant Mouse LF/LTF. No significant cross-reactivity or interference between Mouse LF/LTF and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse LF/LTF concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LF/LTF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LF/LTF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LF/LTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LF/LTF. You can calculate the concentration of LF/LTF in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

54607120

ncbi acc num :

NP_002334.2

ncbi gb acc num :

NM_002343.4

uniprot acc num :

P02788

ncbi mol weight :

73,161 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Latent Infection Of Homo Sapiens With Mycobacterium Tuberculosis Pathway (645267); Mtb Iron Assimilation By Chelation Pathway (1127564); Phagosomal Maturation (early Endosomal Stage) Pathway (645268); Response Of Mtb To Phagocytosis Pathway (1127560)

ncbi summary :

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

uniprot summary :

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted, signal peptide; Protease; EC 3.4.21.-; Secreted. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule. Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; serine-type endopeptidase activity; iron ion binding. Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; activation of NF-kappaB transcription factor; regulation of cytokine production; positive regulation of osteoblast differentiation; retinal homeostasis; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of ATPase activity; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805