ELISA/assay

Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit

MBS2505680

24-Strip-Wells

215 USD

ELISA Kit

Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit

[HNF4a]

[hepatocyte nuclear factor 4-alpha isoform HNF4alpha12; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4-alpha; HNF4alpha10/11/12; TCF-14; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; transcription factor 14; transcription factor HNF-4; hepatocyte nuclear factor 4, alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4]

[HNF4a]

[HNF4A; HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha; HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14]

HNF4A_HUMAN

Rat

392

This kit recognizes Rat HDAC2 in samples. No significant cross-reactivity or interference between Rat HDAC2 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HDAC2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HDAC2 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat HDAC2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HDAC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HDAC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HDAC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat HDAC2. You can calculate the concentration of Rat HDAC2 in the samples by comparing the OD of the samples to the standard curve.

563407074

NP_001274113.1

NM_001287184.1

P41235

43,988 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Developmental Biology Pathway (477129); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1-alpha Transcription Factor Network Pathway (138045); Integrated Pancreatic Cancer Pathway (711360); Maturity Onset Diabetes Of The Young Pathway (83096)

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described. Protein type: Transcription factor; Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding. Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis. Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590