Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit | MyBioSource MBS2505670 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit

catalog :

MBS2505670

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505670

products type :

ELISA Kit

products full name :

Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit

products short name :

[IGF2BP3]

other names :

[insulin-like growth factor 2 mRNA-binding protein 3; Insulin-like growth factor 2 mRNA-binding protein 3; insulin-like growth factor 2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; IGF2 mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; VICKZ family member 3; cancer/testis antigen 98; insulin-like growth factor 2 mRNA binding protein 3; IGF-II mRNA-binding protein 3; KH domain-containing protein overexpressed in cancer; hKOC; VICKZ family member 3]

products gene name :

[IGF2BP3]

other gene names :

[IGF2BP3; IGF2BP3; KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3; IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC]

uniprot entry name :

IF2B3_HUMAN

reactivity :

Human

sequence length :

579

specificity :

This kit recognizes natural and recombinant Human IGF2BP3. No significant cross-reactivity or interference between Human IGF2BP3 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IGF2BP3 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGF2BP3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGF2BP3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGF2BP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IGF2BP3. You can calculate the concentration of IGF2BP3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

30795212

ncbi acc num :

NP_006538.2

ncbi gb acc num :

NM_006547.2

uniprot acc num :

O00425

ncbi mol weight :

21,630 Da

ncbi pathways :

Gene Expression Pathway (105937); Insulin-like Growth Factor-2 MRNA Binding Proteins (IGF2BPs/IMPs/VICKZs) Bind RNA Pathway (161026)

ncbi summary :

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

uniprot summary :

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5 -UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3 -UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Spliceosome; Translation. Chromosomal Location of Human Ortholog: 7p11. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: protein binding; mRNA 3 -UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5 -UTR binding. Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590