catalog number :
MBS2505662
products type :
ELISA Kit
products full name :
Mouse Qa-2 (Qa Lymphocyte Antigen 2 Region) ELISA Kit
products short name :
Qa-2
other names :
Qa-2 cell surface antigen; H-2 class I histocompatibility antigen, Q8 alpha chain; H-2 class I histocompatibility antigen, Q8 alpha chain; minisatellite 10t detected by probe MMS10; histocompatibility 2, Q region locus 8
products gene name :
Qa-2
other gene names :
H2-Q8; H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
uniprot entry name :
HA18_MOUSE
specificity :
This kit recognizes natural and recombinant Mouse Qa-2. No significant cross-reactivity or interference between Mouse Qa-2 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of Mouse Qa-2 is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse Qa-2 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Qa-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Qa-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Qa-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Qa-2. You can calculate the concentration of Qa-2 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
BAA14174.1
ncbi mol weight :
37,411 Da
ncbi pathways :
Adaptive Immune System Pathway (1110669); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (1110688); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (1110689); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83266)