Human Ub (Ubiquitin) ELISA Kit | MyBioSource MBS2505642 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ub (Ubiquitin) ELISA Kit

catalog :

MBS2505642

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505642

products type :

ELISA Kit

products full name :

Human Ub (Ubiquitin) ELISA Kit

products short name :

[Ub]

other names :

[Ubiquitin-63E, isoform D; Polyubiquitin; CG11624 gene product from transcript CG11624-RB; CG11624-PA; CG11624-PB; CG11624-PC; CG11624-PD; Ubi-p63E-PA; Ubi-p63E-PB; Ubi-p63E-PC; Ubi-p63E-PD; Ubiquitin-63-E; Ubiquitin-63E; Ubiquitin-polymeric; magellan; polyubiquitin; ubiquitin 63E; Ubiquitin-63E]

products gene name :

[Ub]

other gene names :

[Ubi-p63E; Ubi-p63E; CG11624; DmelCG11624; DmUb; DmUbi-p63E; magn; Ub; UB; ubi; Ubi-p; ubi-p63E; Ubi-P63e; ubi63E; Ubi63E; Ubi63p; Ubip; Ubip63E; Ubiq; Ubp; Ubi-m]

uniprot entry name :

UBIQP_DROME

reactivity :

Human

sequence length :

763

specificity :

This kit recognizes Human TTR in samples. No Significant cross-reactivity or interference between Human TTR and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000 ng/mL. Sensitivity: 9.38 ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TTR were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TTR were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TTR concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TTR. You can calculate the concentration of Human TTR in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

442630045

ncbi acc num :

NP_001261383.1

ncbi gb acc num :

NM_001274454.1

uniprot acc num :

P0CG69

ncbi mol weight :

85,799 Da

ncbi pathways :

AMER1 Mutants Destabilize The Destruction Complex Pathway (1116754); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1116749); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1116750); APC-Cdc20 Mediated Degradation Of Nek2A Pathway (1115834); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (1115826); APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway (1115835); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (1115836); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (1115831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (1115838); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1115832)

uniprot summary :

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling ().

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590