Mouse DNASE1 (Deoxyribonuclease I) ELISA Kit | MyBioSource MBS2505587 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse DNASE1 (Deoxyribonuclease I) ELISA Kit

catalog :

MBS2505587

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505587

products type :

ELISA Kit

products full name :

Mouse DNASE1 (Deoxyribonuclease I) ELISA Kit

products short name :

[DNASE1]

other names :

[deoxyribonuclease-1; Deoxyribonuclease-1; deoxyribonuclease-1; deoxyribonuclease 1; Deoxyribonuclease I; DNase I; INN: Dornase alfa]

products gene name :

[DNASE1]

other gene names :

[DNASE1; DNASE1; DNL1; DRNI; DNL1; DRNI; DNase I]

uniprot entry name :

DNAS1_HUMAN

reactivity :

Mouse

sequence length :

282

specificity :

This kit recognizes Mouse DKK3 in samples. No significant cross-reactivity or interference between Mouse DKK3 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse DKK3 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse DKK3 were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse DKK3 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DKK3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DKK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse DKK3. You can calculate the concentration of Mouse DKK3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

21361254

ncbi acc num :

NP_005214.2

ncbi gb acc num :

NM_005223.3

uniprot acc num :

P24855

ncbi mol weight :

19,771 Da

ncbi summary :

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. Protein type: EC 3.1.21.1; Deoxyribonuclease; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region; nuclear envelope; nucleus. Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity. Biological Process: apoptosis; DNA catabolic process, endonucleolytic. Disease: Systemic Lupus Erythematosus

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590