catalog number :
MBS2505570
products type :
ELISA Kit
products full name :
Mouse CORT (Corticosterone) ELISA Kit
products short name :
[CORT]
other names :
[cortistatin preproprotein; Cortistatin; cortistatin; cortistatin-14; cortistatin-17; cortistatin-29; prepro-cortistatin; preprocortistatin; cortistatin]
products gene name :
[CORT]
other gene names :
[CORT; CORT; CST-14; CST-17; CST-29]
uniprot entry name :
CORT_HUMAN
specificity :
This kit recognizes Mouse CKMB in samples. No significant cross-reactivity or interference between Mouse CKMB and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CKMB were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CKMB were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse CKMB concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse CKMB. You can calculate the concentration of Mouse CKMB in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001293.3
ncbi gb acc num :
NM_001302.4
ncbi mol weight :
11,532 Da
ncbi summary :
This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]
uniprot summary :
CORT: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs. Belongs to the somatostatin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: extracellular space. Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding. Biological Process: synaptic transmission; G-protein signaling, adenylate cyclase inhibiting pathway
size5 :
10x96-Strip-Wells