Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit | MyBioSource MBS2505556 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit

catalog :

MBS2505556

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2505556

products type :

ELISA Kit

products full name :

Human ECM1 (Extracellular Matrix Protein 1) ELISA Kit

products short name :

[ECM1]

other names :

[extracellular matrix protein 1 isoform 3; Extracellular matrix protein 1; extracellular matrix protein 1; secretory component p85; extracellular matrix protein 1; Secretory component p85]

products gene name :

[ECM1]

other gene names :

[ECM1; ECM1; URBWD]

uniprot entry name :

ECM1_HUMAN

reactivity :

Human

sequence length :

567

specificity :

This kit recognizes natural and recombinant Human ECM1. No significant cross-reactivity or interference between Human ECM1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ECM1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ECM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ECM1. You can calculate the concentration of ECM1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

322302700

ncbi acc num :

NP_001189787.1

ncbi gb acc num :

NM_001202858.1

uniprot acc num :

Q16610

ncbi mol weight :

63,563 Da

ncbi summary :

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

uniprot summary :

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space. Molecular Function: protein C-terminus binding; signal transducer activity; protein binding; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding. Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; positive regulation of endothelial cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; regulation of T-helper 2 type immune response; inflammatory response. Disease: Lipoid Proteinosis Of Urbach And Wiethe

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590