Human ApoA4 (Apolipoprotein A4) ELISA Kit | MyBioSource MBS2505549 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ApoA4 (Apolipoprotein A4) ELISA Kit

catalog :

MBS2505549

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2505549

products type :

ELISA Kit

products full name :

Human ApoA4 (Apolipoprotein A4) ELISA Kit

products short name :

[ApoA4]

other names :

[apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV; Apolipoprotein A4]

products gene name :

[ApoA4]

other gene names :

[APOA4; APOA4; Apo-AIV; ApoA-IV]

uniprot entry name :

APOA4_HUMAN

reactivity :

Human

sequence length :

396

specificity :

This kit recognizes natural and recombinant Human ApoA4. No significant cross-reactivity or interference between Human ApoA4 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 4.688-300ng/mL. Sensitivity: Min: 2.813ng/mL; Max: 300ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ApoA4 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoA4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoA4. You can calculate the concentration of ApoA4 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

71773110

ncbi acc num :

NP_000473.2

ncbi gb acc num :

NM_000482.3

uniprot acc num :

P06727

ncbi mol weight :

45,399 Da

ncbi pathways :

Amyloids Pathway (366238); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

uniprot summary :

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Lipid-binding; Secreted; Endoplasmic reticulum; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: antioxidant activity; lipid transporter activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding. Biological Process: multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; phototransduction, visible light; cholesterol metabolic process; removal of superoxide radicals; lipid homeostasis; neurite regeneration; protein-lipid complex assembly; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; regulation of cholesterol absorption; innate immune response in mucosa; phospholipid efflux; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport; retinoid metabolic process

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805