ELISA/assay

Human FNDC5(Fibronectin Type III Domain Containing Protein 5)ELISA Kit

MBS2505536

48-Strip-Wells

430 USD

ELISA Kit

Human FNDC5(Fibronectin Type III Domain Containing Protein 5)ELISA Kit

FNDC5

fibronectin type III domain-containing protein 5 isoform 2 preproprotein; Fibronectin type III domain-containing protein 5; fibronectin type III domain-containing protein 5; fibronectin type III repeat-containing protein 2; fibronectin type III domain containing 5; Fibronectin type III repeat-containing protein 2Irisin

FNDC5

FNDC5; FNDC5; FRCP2; irisin; FRCP2

FNDC5_HUMAN

Human

212

This kit recognizes natural and recombinant Human FNDC5. No significant cross-reactivity or interference between Human FNDC5 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: The minimum detectable dose of Human FNDC5 is 0.469ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FNDC5 concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FNDC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FNDC5. You can calculate the concentration of FNDC5 in the samples by comparing the OD of the samples to the standard curve.

511094000

NP_715637.2

NM_153756.2

Q8NAU1

15,527 Da

This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p35.1. Cellular Component: peroxisomal membrane; endoplasmic reticulum; integral to membrane; plasma membrane; extracellular region. Molecular Function: hormone activity. Biological Process: response to muscle activity

48-Strip-Wells

430 USD

96-Strip-Wells

520